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. 2016 Oct 13;8:108. doi: 10.1186/s13148-016-0272-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Validation of the top six differentially methylated genes by Sequenom EpiTYPER in MMC patients versus controls. Left: methylation pattern for each CpG unit within the amplicons. Multiple t test was performed for each CpG. *P value <0.05. Right: boxplot representing methylation pattern with box = 25th and 75th percentiles; bars = min and max values. The mean methylation level of each group is shown above the plot. The validation study is performed for 83 MMC patients and 30 controls. *CpGs were first identified by HM450k