Fig. 3.

Demethylation studies and gene expression analysis. Demethylation studies were performed using 5 μM 5-aza-2′-deoxycytidine in HEK cell line. a Sanger sequencing showing demethylation of SOX18 after 5-aza-2′-deoxycytidine treatment. The CpGs from the SOX18 amplicon are annotated below the sequence. b Gene expression analysis of top six genes using qRT-PCR. Standard standard culture conditions, AZA 5-aza-2′-deoxycytidine. *P value <0.05, ***P value <0.001