Figure 4.
Repeated FeTMPyP treatment modulates spontaneous cancer-induced bone pain behaviors and system xc− functional expression in vivo. Animal femurs were injected with breast cancer cells (66.1) or cell-free media (sham) after baseline (presurgery) behavioral measurements. On day 7 after femoral inoculation, 66.1 animals demonstrated bone cancer–induced flinching (A) and guarding (B). FeTMPyP (10 mg/kg, i.p.) or vehicle (saline, 10 mL/kg, i.p.) was administered after behavioral measurements day 7 and continued to day 14 (q.d.). Spontaneous flinching and guarding were assessed before and after treatment on days 10 and 14 (A and B). Femur marrow from inoculated femur (ipsilateral) (C) and contralateral femur (D) was collected from tumor-bearing animals before or after the final treatment on day 14 for glutamate analysis. Day 14 ipsilateral marrow samples from tumor-bearing animals were analyzed for XCT expression using Western blot analysis (E–H); *P < 0.05, **P < 0.01, ***P < 0.001.