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. Author manuscript; available in PMC: 2016 Oct 14.
Published in final edited form as: Messenger (Los Angel). 2015 Jun;4(1):104–111. doi: 10.1166/msr.2015.1045

Figure 3.

Figure 3

NAADP-antagonist BZ194 inhibits Ca2+-signalling and immune synapse formation in TMBP cells stimulated by MBP-pulsed astrocytes. Astrocytes with were pulsed with MBP. Resting rat TMBP cells were incubated with BZ194 (C) or left untreated (B). In control experiments astrocytes were not pulsed with MBP (A). T cells were added to the astrocytes and Ca2+-signalling was measured. Top: Percentage of T cells showing Ca2+-signals above (high-responders) or below (low-responders) 300 nM [Ca2+]i in the first ten minutes after contact to an astrocyte. Bottom: Averaged tracings (±SEM) of T cells showing kinetics of [Ca2+]i, changes in shape index and motility in the first ten minutes after contact to an astrocyte. The majority of T cells is displayed in black, the minority in grey.