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. 2016 Oct 13;12(10):e1006362. doi: 10.1371/journal.pgen.1006362

Fig 3. henji mutants display larger quantal size while maintaining synaptic homeostasis.

Fig 3

(A, B) Representative traces of evoked (A) and spontaneous responses (B) with bar graphs at right for EJP amplitudes of WT, 16.67 ± 1.49 and henji1/1, 14.36 ± 1.81 (A), and mEJP amplitudes of WT, 1.00 ± 0.14; henji1/1, 1.83 ± 0.08 (B). (C-E) Bar graphs show mEJP frequency of WT, 2.26 ± 0.20 and henji1/1, 2.25 ± 0.20 (C), quantal contents calculated as EJP/mEJP, with WT, 12.11 ± 1.24 and henji1/1, 7.82 ± 1.05 (D), and failure analysis at 0.2 mM [Ca2+], with WT, 1.50 ± 0.27; henji1/1, 0.59 ± 0.19 (E). (F-H) Bar graphs show pre- or postsynaptic rescue of henji1/P by UAS-henji for (F) EJPs with C57-GAL4 henji1/+, 22.46 ± 1.13; C57-GAL4 henji1/P, 19.96 ± 2.25; C57>UAS-henji henji1/P, 17.08 ± 0.06; C57>UAS-henji, 18.29 ± 1.58; elav-GAL4 henji1/P, 25.69 ± 5.01; elav>UAS-henji, henji1/P, 27.21 ± 1.56; (G) mEJPs with C57-GAL4 henji1/+, 1.09 ± 0.06; C57-GAL4 henji1/P, 1.48 ± 0.12; C57>UAS-henji henji1/P, 0.68 ± 0.06; C57>UAS-henji, 0.52 ± 0.05; elav-GAL4 henji1/P, 1.51 ± 0.22; elav>UAS-henji, henji1/P, 1.54 ± 0.13; and (H) quantal contents with C57-GAL4 henji1/+, 21.06 ± 1.26; C57-GAL4 henji1/P, 14.29 ± 1.74; C57>UAS-henji henji1/P, 26.36 ± 2.33; C57>UAS-henji, 36.37 ± 3.65; elav-GAL4 henji1/P, 17.51 ± 4.43; elav>UAS-henji, henji1/P, 19.53 ± 1.84. Statistical significance by unpaired Student t-test is shown as * for p < 0.05; ** for p < 0.01; *** for p < 0.001; or n.s. for no significance.