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. 2016 Oct 7;12(10):e1006360. doi: 10.1371/journal.pgen.1006360

Fig 4. Al regulated local up-regulation of YUCs is ethylene dependent.

Fig 4

(A) The expression of YUCp:GFP-GUS transgenes in the root-apex epidermis in presence of Al and either ACC or AVG. Three-day old transgenic YUCp:GFP-GUS seedlings were pre-treated with 1 μM AVG or 1 μM ACC for 1 day, then the seedlings were treated with 1 μM AVG or 1 μM ACC in the presence or not of 25 μM AlCl3 for 2 hours. Cell boundaries appear red following propidium iodide staining. The root- apex TZ is marked by white arrowheads. Scale bar: 100μm. (B) Quantification of the Al-induced fluorescence intensity in the TZ of YUC8p:GFP-GUS and YUC8p:GFP-GUS in (A). Around 30 seedlings were measured in each material. The detected fluorescence region in TZ is marked by yellow rectangles. Cell boundaries appear red following propidium iodide staining. Statistical difference from detected fluorescence is indicated by asterisks (Fisher’s exact test, ***P < 0.001).