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. 2016 Oct 14;12(10):e1006380. doi: 10.1371/journal.pgen.1006380

Fig 1. AS3_9 locus and its enhancer activity in the frontonasal region.

Fig 1

(A) Location of human AS3_9 and surrounding genes. (B) The phastCons conservation track of AS3_9 in mouse (UCSC Genome Browser; mm10); the blue, yellow, and green bars denote the AmnSINE1, X6b_DNA, and MER117 regions, respectively. The construct used to produce AS3_9-lacZ mice is illustrated below. (C) Whole-mount (left), frontal view of the head (top right), and ventral view of the upper jaw (bottom right) showing lacZ expression in AS3_9-lacZ E13.5 embryos. (D) Coronal section of an AS3_9-lacZ E14.5 embryo. (E) ISH for wnt5a in E14.5 wild type. Arrowheads indicate the palatal shelves (C–E). MN, medial nasal process; LN, lateral nasal process; Max, maxillary process; Man, mandibular process. Scale bar (C–E): 0.5 mm.