Fig 3. Functional dissection of AS3_9 enhancer.

Top left: Conservation track from the UCSC Genome Browser (mm10) and TE regions in mouse. Shown are nine constructs (a–j) and their enhancer activity in the maxillary and mandibular processes, nasal processes, and palatal shelves (right). Lateral view (a'–j') and ventral view (a''–j'') of the upper jaw of E13.5 transgenic embryos harboring each deletion construct (a–i) and the construct with a mutated Msx1-binding site (j). LacZ expression was clearly observed in the maxillary and mandibular processes (yellow arrowheads), nasal processes (green arrowheads), and palatal shelves (red arrowheads). Scale bar: 1 mm.