Table 1. Sequences of oligonucleotides used in this study.
name | sequence (5’-3’) |
---|---|
as2b | uag agg uac gug cug agg cTT |
as2bFAM | uag agg uac gug cT*g agg cTT |
D-as2b | TAG AGG TAC GTG CTG AGG CTT |
D-as2bFAM | TAG AGG TAC GTG CT*G AGG CTT |
D-as2b14FAM_21Cy5 | TAG AGG TAC GTG CT*G AGG CTT# |
s2b | gcc uca gca cgu acc ucu aTT |
s2bFAM | gcc uca gca cgu acc ucu aTT* |
D-s2b | GCC TCA GCA CGT ACC TCT ATT |
D-s2bmm | GCC TCA GCA GCG GGA ATA TTT |
Capital letters represent deoxynucleotides. Oligonucleotides used as a guide strand were 5’-phosphorylated.
*position of the C6-linked 5/6-FAM and
#position of Cy5.