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. 2016 Apr 1;2(4):e1501290. doi: 10.1126/sciadv.1501290

Fig. 1. Nuclear TIE2 localization is associated with the resistance of glioma to IR.

Fig. 1

(A and B) Cell viability assay to determine the response to IR of (A) TIE2-expressing GSCs (GSC-13 and GSC-20), TIE2-nonexpressing GSC (GSC-17), and (B) TIE2 isogenic U251 cultures in a time-point experiment. (C) Colony-forming assay of isogenic U251 cells upon IR treatment. (D) TIE2 silencing results in the radiosensitization of GSCs and U251.Tie2 cells. ntsiRNA, nontargeting siRNA. (E and F) TIE2 localizes in the nucleus of U251 cells upon IR, as assessed by (E) immunofluorescence and confocal microscopic analysis and (F) Western blot analysis. (G) TIE2 localizes in the nucleus of GSCs upon in vivo IR of intracranial xenografts. (H) Schematic representation of Tie2 constructs with mutations within the NLS sequence. WT, wild type. (I and J) NLS mutations jeopardize TIE2 (I) nuclear translocation upon IR, and (J) U251 glioma radioresistance. Data represent means ± SD; **P ≤ 0.01, ***P ≤ 0.001. EV, empty vector.