Skip to main content
. 2016 Oct 14;11(10):e0164858. doi: 10.1371/journal.pone.0164858

Fig 4. Tollip mRNA in L-IECs has a shorter poly(A)-tail.

Fig 4

(A) Tollip mRNA in proximal (Pro), medial (Med), and distal (Dis) S-IECs and L-IECs (Lar) of CV mice was analyzed by northern blotting. The blots are representative of three independent experiments. (B) Schematic drawing of oligo DNAs and detection probes used in the assay. (C) The total RNA from medial S-IECs (Med) or L-IECs (Lar) was incubated with oligo DNA, designed as indicated in (B), and RNase H. After 1 h of incubation, the samples were analyzed by northern blotting to detect Tollip mRNA fragments using the illustrated probe. The blots are representative of two independent experiments.