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. 2016 Sep 21;38(5):1443–1449. doi: 10.3892/ijmm.2016.2745

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Copyright: © Zheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Immunohistochemical staining of FERM domain containing 4A (FRMD4A) expression in oral squamous cell carcinoma (OSCC). Original magnification, ×400. Representative images of (A) well-differentiated (score 2), (B) moderately differentiated (score 6), (C) poorly differentiated (score 12), (D) lymph node metastasis (score 9), (E) leukoplakia (score 3) and (F) normal tongue tissues (score 0). The estimated fraction of positive-stained tumor cells was defined as the proportion score (0, none; 1, 10%; 2, 1–50%; 3, 1–80%; 4, >80%), whereas the estimated staining intensity was demonstrated as the intensity score (0, no staining; 1, weak; 2, moderate; 3, strong) with the aggregate score ranging from 0 to 12. Overexpression of FRMD4A was defined as an aggregate score of >4.