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Sequences of primers used for RT-qPCR.
| Gene | Sequence (5′→3′) | Product size (bp) |
|---|---|---|
| β-catenin | TGGTGACAGGGAAGACATCA | 20 |
| CCATAGTGAAGGCGAACTGC | 20 | |
| TCF-4 | CTCCGATGTCCACTTTCCAT | 20 |
| CGCTTCCTCTATTTGCCATT | 20 | |
| Sclerostin | TTCTCCTTCGGGACCTCAAT | 20 |
| TCTCTCACCTCTGCCCATT | 19 | |
| GAPDH | ACAACTTTGGTATCGTGGAAGG | 22 |
| GCCATCACGCCACAGTTTC | 19 |
TCF-4, transcription factor-4; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
