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. 2016 Sep 30;38(5):1490–1498. doi: 10.3892/ijmm.2016.2762

Figure 4.

Figure 4

Effect of the R9 or N-terminal Chk1-binding peptide (Chk1-NP) on cell viability. (A) Viability of NCI-H460 cells was measured by MTT assay following treatment with 0, 5, 10 or 20 µM of R9 or Chk1-NP. Peptide-treated cells were incubated up to 48 h. (B) Viability of HeLa cells was measured by MTT assay following the same treatment as (A). (C) Effect of R9 or Chk1-NP on viability of HeLa cells following genotoxic treatments. Cells were treated with 5 µM of R9 or Chk1-NP for 1 h prior to treatment with genotoxic agents such as 500 nM camptothecin (CPT), 5 mM hydroxyurea (HU) or 10 Gy ionizing radiation (IR). Cell survival was measured by MTT assay after 24 h. Con stands for control. Error bars represent the means ± tandard deviation. The means of 3 independent experiments were graphed. *P<0.05 compared to R9-treated group.