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. 2016 Sep 29;38(5):1359–1366. doi: 10.3892/ijmm.2016.2759

Figure 3.

Figure 3

Apolipoprotein is associated with extracellular vesicles (EVs) purified by ultracentrifugation and P/PEG precipitation from serum, saliva and human liver stem cell (HLSC). (A) Representative western blot analysis of apolipoprotein B100 (ApoB100) and apolipoprotein A1 (ApoA1) associated with EVs isolated by P/PEG precipitation from serum and saliva after gel-filtration with Sephadex G-100 spin columns to remove lipoproteins. Two experiments were performed with similar results. (B) Total RNA extraction from EVs separated from serum and saliva before and after gel-filtration with Sephadex G-100 spin columns to remove lipoproteins. Data are mean ± 1SD of three independent experiments. (C) Representative PCR analysis for ID1 mRNA expressed by serum EVs before 1) and after gel-filtration 2,3) with Sephadex G-100 spin columns to remove lipoproteins. Two experiments were performed with similar results.