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Expression of hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4) after co-culture for 7 days. (A) Western blot analysis of HCN4 protein expression in the transfected groups and neonatal rat ventricular cardiomyocytes (NRVMs). GAPDH was used as the protein control. (B) Quantitative assessment of HCN4 protein levels by integrated optical density analyses. (C) Expression of HCN4 mRNA in the transfected groups. *P<0.05 vs. other groups.
