. 2016 Sep 14;38(5):1403–1410. doi: 10.3892/ijmm.2016.2736

Table I.

PCR primers used in this study.

Gene	Primer	Reaction condition	Product size (bp)
H-TBX18	Sense: 5′-ACGTCATCCGTAAAGACTGTGG-3′	33 cycles at 54°C in 1 mM MgCl₂	251
	Antisense: 5′-AGTCCGTAGTGATGGTCGCC-3′
HCN4	Sense: 5′-AACCTGGGGGCTGGACAGA-3′	33 cycles at 58°C in 1 mM MgCl₂	462
	Antisense: 5-′CTGGGCAGCCTGTGGAGAG-3′
GAPDH	Sense: 5′-GCCATCAACGACCCCTTCAT-3′	33 cycles at 56°C in 1 mM MgCl₂	315
	Antisense: 5′-TTCACACCCATCACAAACAT-3′

H-TBX18, human T-box 18; HCN4, hyperpolarization activated cyclic nucleotide gated potassium channel 4.