Table 1.

GABA-positive cells.

DOI: http://dx.doi.org/10.7554/eLife.17686.002

Cells			anti-GABA	anti-GABA in snf-11(-)	anti-GABA in unc-47(-)	unc-25 knock-in reporter allele	unc-47 fosmid reporter	unc-46 fosmid reporter	snf-11 fosmid reporter	other fast neuro-transmitter
Previously identified (Mclntire et al., 1993b)	Neuronal	RME	+++	+++	+++	+++	+++	+++	+++	none
		RIS	+++	+++	+++	+++	+++	+++	-	none
		AVL	+++	+++	+++	+++	+++	+++	++	none
		DVB	+++	+++	+++	+++	+++	+++	-	none
		DD1-6	+++	+++	+++	+++	+++	+++	-	none
		VD1-13	+++	+++	+++	+++	+++	+++	-	none
Newly identified	Neuronal	RIB	++	++	++	++	+++	++	+	ACh (weak)
		SMDD/V	+/-	+	+	-	+	-	-	ACh
		AVB	++	+	+	-	-	+	-	ACh
		AVA	+/-	+/-	+	-	-	-	-	ACh
		AVJ	++ *	+	+	-	-	-	-	none
		ALA (recycling)	++	-	-	-	+	-	+++	none
		AVF (clearance)	+	-	-	-	-	-	+++	none
	Non-Neuronal	GLR (non-neuronal)	+	-	++	-	-	+	++	none
		hmc (non-neuronal)	++	-	-	-	-	-	+++	none
		muscle	+++	++	++	-	-	-	+++	none
Additional neurons in male		VD12	+++	+++	+++	+++	+++	+++	+++	none
		EF1-4	+++	+++	+++	+++	+++	+++	+++	none
		R2A	+	+	+	-	-	-	-	ACh
		R6A	++	++	++	-	+	-	-	ACh
		R9B	+	+	+	-	-	+/-	-	none

Additional sites of unc-46 and unc-47 expression are shown in Table 2. unc-25 and snf-11 are expressed exclusively in the cells shown here.

Color coding in column 3 (same as in Figure 2E):.

Blue: 'conventional' GABA neurons that synthesize and synaptically release GABA.

Grey: non-conventional GABA neurons which acquire and release GABA by presently unknown means.

Purple: GABA uptake neurons that take up GABA via SNF-11 (based on snf-11 expression and snf-11-dependence of GABA staining).

Green: Non-neuronal GABA uptake cells.

+/- does not consistently stain in all animals. If present, staining is weak.

* Expression as strong as in RIB ('++') in larval stages, but intensity decreases in the adult.