Table 5.
Genetic characterization of tab-1 function in the RME neurons. Expression in RME can not always be unambiguously assigned to the dorsal, ventral, left or right RME neuron, hence only the total number of RME neurons was counted. otEx6804-6806 are tab-1-rescuing arrays (see Material and methods).
| marker | Genotype | Expression observed in: | ||||||
|---|---|---|---|---|---|---|---|---|
| 4 RMEs | 3 RMEs | 2 RMEs | 1 RME | 0 RME | n | |||
| unc-47prom::gfp (otIs509) | wild type | 100% | 0% | 0% | 0% | 0% | 31 | |
| ot796 | 0% | 26.7% | 70% | 3.3% | 0% | 30 | ||
| unc-47prom::gfp (oxIs12) | wild type | 100% | 0% | 0% | 0% | 0% | 30 | |
| gk753 | 16.7% | 30% | 53.3% | 3.3% | 0% | 30 | ||
| unc-25prom::gfp (otIs549) | wild type | 100% | 0% | 0% | 0% | 0% | 30 | |
| ot796/+ | 100% | 0% | 0% | 0% | 0% | 22 | ||
| ot796 | 0% | 23.3% | 76.7% | 0% | 0% | 30 | ||
| gk753 | 0% | 20% | 80% | 0% | 0% | 30 | ||
| ot796 otIs549/+ | 6.7% | 36.7% | 56.6% | 0% | 0% | 30 | ||
| ot796/ok2198 | 0% | 42.8% | 57.2% | 0% | 0% | 28 | ||
| ot796/u271 | 3.3% | 36.7% | 60% | 0% | 0% | 30 | ||
| ot796/gk753 | 3.3% | 36.7% | 60% | 0% | 0% | 30 | ||
unc-46prom::gfp(otIs575) |
wild type | 93.3% | 6.7% | 0% | 0% | 0% | 30 | |
| gk753 | 13.3% | 36.7% | 46.7% | 3.3% | 0% | 30 | ||
| anti-GABA staining | wild type | 70% | 16.7% | 10% | 0% | 3.3% | 30 | |
| ot796 | 10% | 20% | 63.4% | 3.3% | 3.3% | 30 | ||
|
tab-1(gk753); otIs549; Ex[tab-1(+),ttx-3::gfp] |
without otEx6804 | 13.3% | 16.7% | 70% | 0% | 0% | 30 | |
| with otEx6804 | 80% | 8% | 12% | 0% | 0% | 25 | ||
| without otEx6805 | 5% | 25% | 65% | 0% | 5% | 20 | ||
| with otEx6805 | 41.9% | 9.7% | 48.4% | 0% | 0% | 31 | ||
| without otEx6806 | 5.6% | 16.7% | 77.8% | 0% | 0% | 18 | ||
| with otEx6806 | 66.7% | 10% | 23.3% | 0% | 0% | 30 | ||