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GRHL2 expression is increased in Prop1df/df mice at p5. GRHL2 expression is mainly detected in the cleft cells of the anterior lobe (AL) and intermediate lobe (IL) cells of control pituitaries (A and C). In Prop1df/df pituitaries, the number of GRHL2 positive cells appears to be increased in the AL cleft (B and D). Grhl2 mRNA levels are significantly increased in Prop1df/df mice pituitaries compared to controls (E). *, P < 0.05. n=3 (immunohistochemistry). n=3–4 (qRT-PCR).
