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. Author manuscript; available in PMC: 2017 Oct 14.
Published in final edited form as: Circ Res. 2016 Sep 13;119(9):e129–e138. doi: 10.1161/CIRCRESAHA.116.308263

Figure 5. iNOS binds and S-nitrosylates Ring1A.

Figure 5

BJ fibroblasts were treated with vehicle; PIC; or with PIC and the NFκB inhibitor Bay117082 for 3 days and proteins were collected for western blot or immunoprecipitation application. (A) Western blot detection of iNOS and SNO protein expression. (B) Proteins from cell lysate were immunoprecipitated Online with SNO or control IgG antibody and subject to western blot detection of RING1A. (C) Proteins from cell lysate were immunoprecipitated with RING1A or control IgG antibody and subject to western blot detection of SNO protein. (D) Proteins from cell lysate were immunoprecipitated with RING1A or control IgG antibody and subject to western blot detection of iNOS protein. Data are shown as the means ± SEM and are representative of 3 independent experiments.