Figure 8. Innate immune activation decreases RING1A binding to chromatin and reduces repressive histone markers.
BJ fibroblasts treated with or without PIC for three days during the transdifferentiation protocol. (A) Western blot examination of iNOS, H3K27me3, RING1A and SNO protein expression. (B) RING1A, (C) H3K27me3 and (D) H3K9me2 binding to the CD144 promoter were analyzed by ChIP-PCR. *P<0.05, vs vehicle treated-CT. Data are shown as the means ± SEM and are representative of 3 independent experiments.