Figure 1.

Rapid Decrease in Parental H3 Histone Density in UVC-Damaged Chromatin Regions

(A) Left: current model for histone dynamics in UVC-damaged chromatin (adapted from Adam et al., 2015). The incorporation of new histones (green) raises questions about the fate of parental histones (red). Right: experimental strategy for tracking parental histone dynamics at DNA damage sites.

(B) Distribution of parental histones H3.3 (red) at the indicated time points after UVC laser damage in U2OS cells stably expressing H3.3-SNAP and GFP-XPC.

(C) Dynamics of parental H3.3 (red) at early time points after local UVC damage in U2OS H3.3-SNAP cells. White arrowheads, illuminated areas. Scale bars, 10 μm. Red fluorescence measured in irradiated areas is normalized to before laser. Error bars, SD from n cells scored in two independent experiments. Labeling parental histones in green instead of red gave similar results (data not shown).

See also Figures S1 and S2 and Movie S1.