Figure 5.
Parental H3.3 Redistribution Is Independent of New H3.3 Deposition
(A) Dynamics of parental (red) and new H3.3 (green) at the indicated time points after UVC laser damage in U2OS H3.3-SNAP cells. Red and green signals measured in damaged areas are normalized to before laser. Error bars, SD from n cells scored in two independent experiments.
(B) Distribution of parental and new H3.3 as in (A) in cells treated with the indicated siRNAs (siLUC: control). HIRA knockdown is verified by western blot and by the inhibition of new H3.3 deposition at damage sites.
(C) Distribution of parental and new H3.3 as in (B). siRNA efficiency was verified by qRT-PCR. Error bars, SD from two independent experiments. Red fluorescence loss is measured in damaged areas at 15 min compared to before laser (n cells scored in two independent experiments). White arrowheads, irradiated areas. Scale bars, 10 μm.