Table 2.
Development of in vitro produced bovine embryos co-cultured in the presence of two concentrations of adipose tissue-derived mesenchymal stem cells (b-ATMSCs) or cultured with the addition of conditioned medium
| Groups | No. of oocytes | Cleaved (%) | Blastocysts (%) |
|---|---|---|---|
| SOF | 63 | 34 (53.9) a | 12 (19.0) a |
| SOF-Cond | 72 | 58 (80.5) b | 18 (25.0) a |
| 103 b-ATMSCs | 61 | 48 (78.6) b | 20 (32.7) a |
| 104 b-ATMSCs | 61 | 51 (83.6) b | 28 (45.9) b* |
Different lowercase letters within a column denote significant differences (ANOVA; p < 0.05). The mean percentage was calculated from four independent experiments and showed normal distribution (Shapiro-Wilk test)
SOF synthetic oviductal fluid (control), SOF-Cond SOF preconditioned in culture with b-ATMSCs for 48 h, 10 3 or 10 4 b-ATMSCs SOF + co-culture with 103 or 104 cells/mL seeded 24 h earlier
*Comparison with 103 b-ATMSCs showed a p value of 0.051