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. 2015 Nov 2;90(11):2745–2761. doi: 10.1007/s00204-015-1621-7

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Performance of primer efficiency. Examples of the amplification curves from the calibration performed with Fluidigm dynamic array for a GAPDH, b JUN and c SIRT2. Six serial dilutions (1–200-fold) of standard cDNA are shown. Plot of calibration curves (x-axis log₁₀ of relative template concentration, y-axis C _q value of template concentration) with linear regression trend line and correlation coefficient for d GAPDH, e JUN and f SIRT2. Primer efficiency can be calculated from the slope of the calibration curve