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. 2016 Oct 13;7:13181. doi: 10.1038/ncomms13181

Figure 5. LWD1 is the co-activator of TCP20 for the expression of CCA1 at dawn.

Figure 5

(a) pCCA1::LUC2 expression is increased in TCP20-overexpressing lines. (b) Overexpression of TCP20 fails to increase the expression of CCA1 promoter in lwd1 lwd2. Data are mean±s.e. (n=6–8 seedlings for each independent line; see the corresponding data for TCP22ox in Supplementary Fig. 9a,b). Similar results were observed in three independent experiments. (c) TCP20/TCP22 associate with the CCA1 promoter in vivo independent of LWDs. Asterisk indicates that overexpressed TCP20/TCP22 in WT or lwd1 lwd2 backgrounds preferentially binds with amplicon ‘b' (Student's t test; *P<0.005). (d) LWD1 associates with CCA1 promoter independent of TCP20/TCP22. Data are shown as mean±s.d. from one representative experiment (n=3 technical replicates). Asterisk indicates that LWD1 preferentially binds to the amplicon ‘b' in both WT or tcp20 tcp22 (Student's t test; *P<0.001). Similar results were observed in three independent experiments. (e) A diagram of LWD1 and TCP20/TCP22 co-activating the expression of CCA1.