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. 2016 Oct 13;7:13096. doi: 10.1038/ncomms13096

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Copyright © 2016, The Author(s)

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(a) Kaplan–Meier survival analysis of Csf1r.iCre;Porcn^fl/fl and WT mice exposed to WBI (9.4–12.4 Gy). Csf1r.iCre;Porcn^fl/fl mice show reduced survival following lethal doses of WBI (11.4–12.4 Gy WBI) with 100% mortality within 7–12 days of radiation exposure, compared with WT that have 60% survival beyond 15 days post WBI (P<0.0001, P<0.004 Log-rank (Mantel–Cox) test; n=15 per group). No significant survival difference between Csf1r.iCre;Porcn^fl/fl or WT littermate mice was observed with the irradiation dose of 9.4–10.4 Gy WBI (n=15 per group). (b) Body weight of mice at post irradiation time points (11.4 Gy and 12.4 Gy WBI). (c) Complete blood count (CBC) analysis. CBC measurements for Csf1r.iCre;Porcn^fl/fl and WT mice exposed to 0 Gy/11.4 Gy/12.4 Gy WBI. Blood samples were drawn at 5 days post irradiation (n=3 per group). (d) HE staining of jejunum section from Csf1r.iCre;Porcn^fl/fl and WT mice exposed to 0 Gy/11.4 Gy WBI at day 5 post irradiation. No significant differences in crypt villus structure were noted in non-irradiated Csf1r.iCre;Porcn^fl/fl mice compared with WT mice. Note, shortening of crypt depth as well as loss of crypts in Csf1r.iCre;Porcn^fl/fl mice exposed to irradiation (n=3 per group) compared with WT mice. (e) Histogram demonstrating crypt depth (μM) from jejunal sections of Csf1r.iCre;Porcn^fl/fl and WT mice exposed to 0 or 11.4 Gy WBI. Both WT and Csf1r.iCre;Porcn^fl/fl mice exposed to irradiation showed reduction in crypt depth at day 5 post irradiation compared with un-irradiated control WT mice *P<4.66E−05, Csf1r.iCre;Porcn^fl/fl mice *P<9.63E−08. However, loss of crypt depth was significantly greater in Csf1r.iCre;Porcn^fl/fl mice compared with WT mice *P<8.84E−07 unpaired t-test, two-tailed. (f) Histogram demonstrating the number of crypts mm⁻¹ from jejunal section of Csf1r.iCre;Porcn^fl/fl and WT mice exposed to 0 or 11.4 Gy WBI. Both WT and Csf1r.iCre;Porcn^fl/fl mice exposed to irradiation showed reduction in crypt number at day 5 post irradiation compared with un-irradiated control WT mice *P<0.0008, Csf1r.iCre;Porcn^fl/fl mice *P<1E−08. However, loss of crypt was significantly greater in Csf1r.iCre;Porcn^fl/fl mice compared with WT mice *P<9.79E−09 unpaired t-test, two-tailed. (g) Histogram demonstrating villi length from jejunal section of Csf1r.iCre;Porcn^fl/fl and WT mice exposed to 0 or 11.4 Gy WBI. Both WT and Csf1r.iCre;Porcn^fl/fl mice exposed to irradiation showed reduction in crypt number at day 5 post irradiation compared with un-irradiated control WT mice*P<0.0006, Csf1r.iCre;Porcn^fl/fl mice *P<8.9E−08. However, loss of villi length was significantly greater in Csf1r.iCre;Porcn^fl/fl mice compared with WT mice *P<7.98E−09 unpaired t-test, two-tailed.