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. 2016 Oct 13;7:13096. doi: 10.1038/ncomms13096

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Copyright © 2016, The Author(s)

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(a) Experimental design and Kaplan–Meier survival analysis of Csf1r.iCre;Porcn^fl/fl mice (n=10 per group) receiving CM (i.v.) derived from WT/Porcn-null BMMΦ at 1 and 48 h post WBI (11.2 Gy). Mice receiving WT BMMΦ CM showed 40% survival (P<0.003 Log-rank (Mantel–Cox) test) beyond 25 days compared with mice receiving Porcn-null BMMΦ CM or untreated mice where 100% of mice died within 12 days after irradiation. (b) Representative HE and BrdU immunohistochemistry of mice jejunal sections. Note, restitution on crypt villus structure with the increase in crypt cell proliferation in Csf1r.iCre;Porcn^fl/fl mice receiving WT BMMΦ CM compared with Porcn-null BMMΦ CM treatment. (c) The proliferation rate was calculated as the percentage of BrdU-positive cells over the total number of cells in each crypt and displayed as bar diagrams. Crypt cell proliferation rate in irradiated mice: WT BMMΦ CM versus Porcn-null BMMΦ CM treatment group *P<2.21E−07 (n=5 per group), WT BMMΦ CM versus αMEM growth medium treatment group *P<2.69E−07 (n=5 per group; unpaired t-test, two-tailed). (d) Histogram showing number of crypts mm⁻¹ in jejunal sections of Csf1r.iCre;Porcn^fl/fl mice. Irradiated Csf1r.iCre;Porcn^fl/fl mice receiving WT BMMΦ CM showed less crypt loss compared with mice receiving Porcn-null BMMΦ CM or αMEM growth medium (*P<6.86E−09 and *P<6.74E−08 unpaired t-test, two-tailed). (e) Histogram showing villus length in jejunal sections of Csf1r.iCre;Porcn^fl/fl mice. Irradiated Csf1r.iCre;Porcn^fl/fl mice receiving WT BMMΦ CM showed less reduction in villi length compared with mice receiving Porcn-null BMMΦ CM or αMEM growth medium (*P<3.74E−06 and *P<3.60E−06 unpaired t-test, two-tailed). (f) Histogram demonstrating serum dextran level in Csf1r.iCre;Porcn^fl/fl mice. Mice receiving WT BMMΦ CM showed a lower serum dextran level thereby suggesting restitution of epithelial integrity compared with mice receiving Porcn-null BMMΦ CM or αMEM growth medium (*P<0.006 and *P<0.009 respectively; n=3 per group). Untreated mice also showed a lower serum dextran level compared with irradiated mice receiving αMEM growth medium (P<0.003) or Porcn-null BMMΦ CM (P<0.001; unpaired t-test, two-tailed).