Figure 3.

The Consequences of SUN5 Mutations on Protein Distribution in Sperm

SUN5 immunofluorescence in sperm from control (WT) and affected individuals. SUN5 staining is confined to the head-tail junction region (exactly in the distal part of the inner nuclear membrane, which is close to the distal end of the nucleus) in control sperm (highlighted by white arrow). This specific pattern of distribution is not observed in abnormal head-tail junction spermatozoon of affected individuals with SUN5 mutations. Anti-α-tubulin antibody staining paints the tails of the sperm, and DAPI indicates the nucleus in the sperm head. The scale is shown at the bottom left panel. (Inset) Digital enlargement of the respective sperm head-tail junction regions.