Figure 5.

Expression of Drosophila ATAD3A Ortholog bor with p.Arg534Trp Leads to a Decrease in Mitochondria in Motor Neurons

(A) Effects of expression of wild-type bor and bor^R534W, driven by different Gal4 drivers, on viability.

(B) Confocal micrographs of ventral nerve cord (VNC), axons, and boutons from third instar larvae carrying D42-Gal4 and UAS-mito-GFP (green) together with UAS-empty (control), UAS-bor^WT, or UAS-bor^R534W. Neurons are labeled by HRP (blue) and boutons by Dlg (red).

(C) Quantification of mitochondrial density in VNC neuropil in control, UAS-bor^WT, or UAS-bor^R534W larvae carrying D42-Gal4, UAS-mito-GFP together with the respective vectors.

(D and E) Quantification of mitochondrial volume in axons (D) and in boutons (E) of larvae carrying D42-Gal4, UAS-mito-GFP together with UAS-empty, UAS-bor^WT, or UAS-bor^R534W.

(C–E) Error bars indicate standard error of the mean (SEM). p values were calculated by Student’s t test. ^∗p < 0.05, ^∗∗∗p < 0.001. N.S. indicates not statistically significant.