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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Mol Cancer Res. 2016 Jun 29;14(10):909–919. doi: 10.1158/1541-7786.MCR-15-0474

Figure 3.

Figure 3

MiR-221/222 sponge ALL cells have increased p27, with reduced proliferation and chemosensitivity. A, Schematic of lentiviral miRNA sponge construct along with western blot of p27 abundance alterations within Nalm-27 and REH miR-221/222 sponge cells. Values depict fold change relative to empty vector control and normalized to housekeeping gene GAPDH. B, Immunofluorescent images of p27 in miR-221/222 sponge cells compared to empty vector control. C, Proliferation index was measured 4 days after cell retention dye addition in cells described in A. D, The viability of these cells after 48 hour exposure to Ara-C and VCR were measured by trypan blue exclusion. Error bars represent standard error of the mean with samples performed in triplicate. *, P < 0.05. Western blot, immunofluorescent images and bar graphs are representative data from 3 independent experiments. Scale bars, 10 μm. EV, empty vector; Sp, sponge; Ara-C, cytarabine; VCR, vincristine.