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. Author manuscript; available in PMC: 2017 Oct 15.
Published in final edited form as: Cancer Res. 2016 Aug 8;76(20):5926–5932. doi: 10.1158/0008-5472.CAN-16-0800

Fig. 3. Hsp70 knockout in host cells suppresses infiltration of macrophages in tumors.

Fig. 3

A. Hsp70 knockout in host suppresses macrophage infiltration into the tumor cell injection sites. WT and Hsp70KO mice were injected s.c. with 106 B16 melanoma cells in matrigel. On day 5 post-injection, the material at the injection site was collected, sliced and stained for the presence of macrophages with F4/80 antibodies Macrophages are in brown color and their number was quantified as in Fig. 2A. B and C. Hsp70KO suppress migration of macrophages. Bone marrow macrophages were isolated from WT and Hsp70KO mice and their migration was monitored by the “wound healing” (B) and transwell (C, left panel) assay as described in Fig. 2E. C. Left panel – picture of cells migrated in transwell assay, right panel – quantification of the transwell migration. Data shown are means+/SEM of triplicates.