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. Author manuscript; available in PMC: 2017 Oct 23.
Published in final edited form as: J Mol Biol. 2016 Aug 26;428(21):4280–4297. doi: 10.1016/j.jmb.2016.08.021

Fig. 4. LC variable domains form amyloid-like fibrils under native-like conditions, while full-length LCs form amorphous aggregates at high temperature.

Fig. 4

Representative electron micrographs of aggregates formed by 20 μM ALLC-V (a) and 20 μM JTO-V (b) at 37 °C, 250 rpm in PBS, pH 7.4 for 48 h. Aggregates formed by ALLC-FL (c) and JTO-FL (d) at 55 °C in PBS, pH 7.4 for 96 h are amorphous. Scale bars = 0.2 μm. Both conditions result in the formation of amorphous aggregates, but only the V-domains additionally form amyloid fibrils.