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. Author manuscript; available in PMC: 2017 Oct 23.
Published in final edited form as: J Mol Biol. 2016 Aug 26;428(21):4280–4297. doi: 10.1016/j.jmb.2016.08.021

Fig. 6. Folding and unfolding rates of ALLC and JTO.

Fig. 6

(a) Unfolding rates of ALLC-FL (black) and JTO-FL (green) as a function of urea concentration. The change in fluorescence emission at 350 nm over time was fitted to an exponential equation and the observed rate extracted. Representative transients (gray) and fits (colored as for Fig. 1) are shown on the right. In the case of JTO-FL the large amplitude rate from a double-exponential fit was used to account for the minor lag phase present at low urea concentrations. The logarithm of the rates versus urea concentration was fitted to a linear equation [59]; for JTO-FL the rate associated with the large amplitude change was used. The unfolding rate in the absence of urea, kuH2O, is the y-intercept of the fit, and the denaturant dependence of the unfolding rate, mu, is its slope. (b) Folding and unfolding kinetics of ALLCV (red) and JTO-V (blue), analyzed as for (a) except that the data were fitted to a 2-state kinetic folding model [59]. Representative transients and fits are shown on the right. Parameters are shown in Tables 1 and 2.