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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Ocul Surf. 2016 Aug 12;14(4):484–494. doi: 10.1016/j.jtos.2016.08.001

Figure 2.

Figure 2

Brightfield (A and B) and PPARγ immunostaining (C and D) of primary cultured mouse meibocytes (A and C) and SV40 immortalized CN-G2 meibocytes (B and D). Note that both cells in culture appear to contain prominent vacuoles (arrows) and show cyotplasmic and nuclear PPARγ staining. Cytoplasmic vacuoles showed strong staining with fluorescent LipidTox (E) indicating the presence of neutral lipids. Area of LipidTox staining was then thresholded (F, orange area) to measure lipid area. Each image is representative of 3 separate coverslips.