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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Bioorg Med Chem. 2016 Sep 12;24(21):5618–5625. doi: 10.1016/j.bmc.2016.09.028

Figure 3. Sulfydryl inhibition of anti-MA quinolinequinone activity.

Figure 3

HIV-1 MA-RNA electrophoretic mobility assays were performed as described in Figure 2 to monitor free (F) and MA-bound (B) Sel15 RNA oligonucleotide. In the lefthand lane, MA (15 µM) was preincubated with DMSO prior to the RNA (15 µM) binding incubation. In the righthand lanes, MA was preincubated with 50 µM 81047 alone (second lane from the left), or 50 µM 81047 plus 200 µM glutathione (GSH), cysteine (Cys), β-mercaptoethanol (βME), dithiothreitol (DTT) or Tris(2-carboxyethyl)phosphine hydrochloride (TCEP).