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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Bioorg Med Chem. 2016 Sep 12;24(21):5618–5625. doi: 10.1016/j.bmc.2016.09.028

Figure 8. Effects of 81047 on HIV-1 replication.

Figure 8

Human CEM T cells were treated with DMSO or the indicated concentrations of 81047 and infected with the NL4-3 strain of HIV-1. At 7 d post-infection, cell samples were collected, proteins were fractionated by electrophoresis. Actin levels (bottom panel) and HIV-1 PrGag, p41 and capsid (CA) levels (top panel) were determined by immunoblotting. Quantitation indicated that 10 µM 81047 treatment reduced protein levels (as percentages of untreated samples) as follows: PrGag, 17%; p41, 5%; CA, 18%; actin, 14%. Quantitation of 1 µM 81047 treatment levels was as follows: PrGag, 36%; p41, 35%; CA, 54%; actin, 83%.