Table 1. Quinolinequinone activities.

Compounds are as shown in Figure 1, and listed in accordance with their NSC numbers. Percentage RNA binding inhibition values were determined in electrophoretic mobility gel shift assays with 15 µM MA, 15 µM Sell5 RNA ligand, and 100 µM compounds, as shown in Figure 2. Percentage glutathione (GSH) inactivation values were determined by measurement of GSH free sulfhydryl levels after incubation of 200 µM GSH with 200 µM compound for 1 h at 25°C. Percentage MA crosslinking values were determined after 2 h, 25°C incubations of 30 µM MA with 50 µM compound, and measurement of dimer and monomer bands as shown in Figure 5. Fifty percent cytotoxic (CC50) concentrations (in µM) are shown for human embryonic kidney HEK293T (293) cells and for the human T cell lines CEM-SS (CEM) and MT4. Values were determined by MTS assay at 2 d post-treatment with compounds. Values are averages from three (GSH inactivation) or two (all other assays) independent experiments, with standard deviations as shown.

Compound	%RNA binding	% GSH	% MA	CC50 values (µM)
	inhibition	inactivation	X-linking	293	CEM	MT4
76882	69±26	96±1	47±17	8±6	0.6±0.1	0.7±0.1
76883	36±16	33±12	10±2	22±20	2±0	2±0
76885	22±16	54±23	6±1	7±3	2±0	1±1
76886	28±1	95±0	10±4	8±2	6±0	2±0
76893	26±1	50±9	13±5	9±2	2±1	2±0
81046	21±6	92±3	16±11	9±1	2±0	4±3
81047	99±2	95±3	63±6	17±4	2±1	1±1
81048	50±11	50±4	29±1	18±0	5±0	3±4
81050	39±30	96±2	13±10	5±4	0.7±0.4	1±1
81056	26±13	53±6	11±6	4±3	0.9±0.2	1±1
82130	21±13	3±3	6±1	ND	ND	ND
84999	76±6	30±4	20±13	7±2	5±0	3±1
102729	73±1	56±1	51±11	18±2	3±0	1±1
105808	31±1	96±1	10±2	4±2	1±0	0.6±0.6
663284	3±3	46±5	8±2	13±5	6±1	2±0