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. 2016 Oct 15;6(2):223. doi: 10.1007/s13205-016-0539-y

Table 1.

Various genes targeted for the mRNA expression and primers designed for their amplification to study the effect of vanillin on their expression level

Gene ID Gene Primer
Antibiotic synthesis genes
 SCO5877 redD RT_redD_F: CCCGACAACGTCCTCAAC
RT_redD_R: CGAGACGAGTCTCAGGAAGC
 SCO5085 actII-orf4 RT_actII4_F: AGAATAGGGCCGATGATTCC
RT_actII4_R: CCCAGTTCGTCGGACAGTAT
Morphological genes
 SCOt24 bldA RT_bldA_F: GCCCGGATGGTGGAATGCAG
RT_bldA_R: TGGTGCCCGGAGCCGGACTT
 SCO3323 bldN RT_bldN_F: CCTCGAGTCCCTCTCCAAC
RT_bldN_R: CGGTACTGGAGCGTTTTGAT
 SCO3926 ssgA RT_ssgA_F: CCTTTCATCTGCCCGGAGAC
RT_ssgA_R: CGACCTGAAGTCGGATCAGC
 SCO1541 ssgB RT_ssgB_F: TCGTGTGCATCGCTCTCAG
RT_ssgB_R: CTAGCTTTCCGCCAGGATGT
 SCO3925 ssgR RT_ssgR_F: GGCTGTTCTTCCTCGGTGAG
RT_ssgR_R: GAGACGCACATGACCTCGAT
 SCO2082 fstz RT_ftsZ_F: GTTCATCGCCATCAACACCG
RT_ftsZ_R: TGTCACGAAGACCATGTCGG
Quorum sensing and pleiotropic genes
 SCO6266 scbA RT_scbA_F: ACTACACCTGCCACCTCGAC
RT_scbA_R: GCCGGTAGACTTGAGGACTG
 SCO6265 scbR RT_scbR_F: TCTTCGAGAAGCAGGGCTAC
RT_scbR_R: GCCCATGTCGATGAGTTCTT
 SCO4425 afsS RT_afsS_F: ATGAGCGACAAGATGAAGGA
RT_afsS_R: GGTTGTCCATCGTGGTGAT
 SCO4426 afsR RT_afsR_F: GGCTGCTGGACTTCTACCTG
RT_afsR_R: CCTCCGTGTACAGCCAGTC