FIGURE 1.

Characterization of JcWRKY transgenic tobacco plants. (A) Schematic representation of the pCAMBIA1301 – 35S: JcWRKY construct used for Nicotiana tabacum transformation. (B) GUS assay of T₀ leaf and T₁ seedlings showing positive expression in the transgenic lines and negative expression in WT plants. PCR confirmation of transgenic lines (lane number denote transgenic line number) with (C) hptII (964 bp) and (D) JcWRKY (693 bp) primers. (E) Reverse transcriptase PCR of WT and JcWRKY transgenics. Amplification was observed with some transgenics, while no amplification with WT. Actin gene was used as an internal control. M refers to 100 bp plus ladder (MBI Fermentas). (F) GUS: NRA ratio for determination of copy number of transgene in tobacco plants.