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Scheme of the method used to measure plasmid stability of S. Typhimurium pSLT derivates. A cassette containing a kanamycin resistance gene (aph) and the DNA gyrase inhibitor ParE-encoding gene was used to disrupt genes of interest (top). Grown cultures were plated in rhamnose-containing agar plates and synthesis of ParE toxin was induced. Cells that kept pSLT plasmid and therefore aph-parE cassette, were selectively killed (bottom).
