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. 2016 Oct 17;3:66. doi: 10.3389/fmolb.2016.00066

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Copyright © 2016 Lobato-Márquez, Molina-García, Moreno-Córdoba, García-del Portillo and Díaz-Orejas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Plasmid stability assays of S. Typhimurium pSLT derivates. Segregants fraction measurement of pSLT plasmid comparing bacteria bearing the following plasmids: (i) wild type pSLT, (ii) pSLT lacking ccdAB_ST TA module, (iii) pSLT lacking vapBC2_ST TA system, and (iv) pSLT lacking parAB partition system. Disruption of all maintenance modules significantly decreased pSLT stability. The fraction of segregants was determined dividing the number of colony forming units grown in M9-rhamnose-agar plates (plasmid-free bacteria) by total bacteria grown in LB-agar plates. Data represent the means and standard deviations from five independent experiments. ^*P < 0.05; ^***P < 0.001 by one-way ANOVA and Tukey's multiple comparison post-test.