Figure 5.

Genetic organization and transcriptional analysis of the ccdAB_ST locus present in S. Typhimurium pSLT plasmid. (A) Genetic organization of ccdAB_ST in S. Typhimurium pSLT. Black arrows represent the location and orientation of the genes (scaled). Under the scheme it is marked pSLT DNA sequence coordinates. Primers used for RT-PCR (number 4) and cDNA amplification (numbers 1–4) are denoted above. (B) Co-transcription analysis of ccdAB_ST and downstream genes. cDNA was synthesized using a primer annealing with PSLT032 (named as 4). Primers named as 1, 2, 3, and 4 were used in combination to a primer annealing in 3′-end of ccdB_ST (dashed arrow) to PCR-amplify each region, and PCR products were resolved in a 0.8% agarose gel. DNA marker is shown on the left. The expected sizes of PCR-amplified DNA fragments are indicated with arrows on the left. All genes are co-transcribed with those encoding the ccdAB_ST TA system.