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. 2016 Oct 17;9:98. doi: 10.3389/fnmol.2016.00098

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Copyright © 2016 Kobeissy, Hansen, Neumann, Fu, Jin and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neuronal and glial differentiation from embryonic NSCs is not affected by Emx1 gene deletion. NSCs differentiate in response to growth medium containing differentiation supplement after 7–14 days. Representative photomicrographs showing undifferentiated neural stem cells grown as monolayer (Nestin; A, soon after seeding; B, 7 days after seeding), differentiated neurons (C, TuJ1) and glia (D, GFAP) in red and nuclei in blue (Hoechst). Bar graph indicated the quantitative results for differentiation. The extent of neuronal vs. glial differentiation was determined by counting 200 cells after staining with antibodies against TuJ1 and GFAP. No differences in the differentiation ratios of neurons and astrocytes between Emx1^+/+ and Emx1^−/− group (E). Scale bar, 25 μm. N = 4 NSC samples/group, and each NSC sample was plated in 3–4 wells.