Table 3.

Antibacterial activity tested against E. coli of the eight ethyl acetate fractions separated using silica gel column chromatography and determined using the agar well diffusion method. Each value is the mean ± standard deviation from three replicates.

Fraction (2 mg/wella)	Inhibition zone (mm)b
Fraction 1	22 ± 1
Fraction 2	26 ± 1
Fractions 3–8	—
Chloramphenicolc	27 ± 2

^a2 mg of dried fraction was dissolved in 100 μL of 15% methanol and the whole volume added to the well.

^bInhibitory zones in mm, including diameter of the well (6.0 mm). —: no inhibition.

^c15 μL of 10 mg/mL was added to each well.