Figure 9.

Kinetics of IFN-γ TNF-α IL-2 and IFN-γ TNF-α responses by Tcm. For long-term culture, PBMCs isolated at 6 weeks post-vaccination (6 WPV), 3 weeks post-infection (3 WPI) and 8 WPI were stimulated with a cocktail of Ag85A, TB10.4, and PPD for 13 days followed by transfer to 96-well round bottom plates with APCs and addition of media alone, Ag85A/TB10.4 or ESAT-6:CFP10 for an additional 16 h. Cytokine production and effector/memory phenotype were determined by ICS flow cytometry. Results are presented as average and SE. Percentages of long-term cultured Tcm cells (gated on CD45RO⁺/CCR7⁺/CD4⁺ cells) producing IFN-γ/TNF-α/IL-2 or IFN-γ/TNF-α upon recall stimulation with Ag85A/TB10.4 (A,B) or ESAT-6:CFP10 (C,D). *Differences (P < 0.05, Tukey’s) in the cytokine production by non-vaccinates and vaccinates.