Table 1.
Primary and secondary monoclonal antibodies and staining reagents.
| Reagent | Specificity, source | Fluorophore, source |
|---|---|---|
| ILA11 | Bovine CD4, Washington State University | PE-Cy7, SouthernBiotech |
| ILA116 | Bovine CD45RO, Washington State University | APC-Cy7, Life Technologies |
| 7D12 | Human CCR7 (CD197), BD Pharmingen | AF 350, Life Technologies or Spectral Red, SouthernBiotech |
| MCA1783-PE | Bovine IFN-γ, AbD Serotec | Not applicable |
| MCA2334-FITC | Bovine TNF-α, BD Pharmingen | Not applicable |
| AbD14386-DyLight649 | Bovine IL-2, AbD Serotec | Not applicable |
| Live/dead staining | Pacific Blue, Life Technologies | Not applicable |