. 2016 Oct 17;7:421. doi: 10.3389/fimmu.2016.00421

Table 2.

IFN-γ/TNF-α/IL-2 and IFN-γ/TNF-α are the predominant polyfunctional profile in response to M. bovis infection, as assessed by long-term and ex vivo assays.

Cytokine profile^c	PPD		ESAT6:CFP10
	Long-term Average (SEM)	Ex vivo Average (SEM)	Long-term Average (SEM)	Ex vivo Average (SEM)
	IFN-γ/TNF-α/IL-2	3.92 (0.55)^a	4.03 (0.78)^a	4.51 (0.64)^a	3.79 (0.71)^a
	IFN-γ/TNF-α	4.44 (0.51)^a	4.69 (0.59)^a	5.37 (0.59)^a	4.32 (0.70)^a
	IFN-γ/IL-2	1.31 (0.09)^b	1.26 (0.22)^b	1.01 (0.36)^b	1.18 (0.24)^b
TNF-α/IL-2	2.02 (0.24)^b	2.09 (0.24)^b	1.39 (0.14)^b	2.06 (0.30)^b
IFN-γ	1.15 (0.12)^b	1.27 (0.35)^b	0.86 (0.13)^b	1.28 (0.41)^b
TNF-α	0.31 (0.05)^b	1.08 (0.23)^b	0.20 (0.06)^b	1.15 (0.28)^b
IL-2	0.98 (0.36)^b	1.83 (0.29)^b	1.48 (0.35)^b	1.79 (0.34)^b

^a,bDifferent letters indicate differences (P < 0.05) in the frequency of profiles within each culture condition (long-term or ex vivo). Differences in polyfunctional profile frequencies between long-term and ex vivo were not detected (P > 0.05, Tukey’s).

^cData are presented as frequencies (%) of cytokine-producing cells within long-term or ex vivo cultures.