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. 2016 Oct 17;7:427. doi: 10.3389/fimmu.2016.00427

Figure 3.

Figure 3

Cytokine/chemokine production in splenocyte culture supernatants. C57BL/6 mice were subcutaneously immunized with H56 alone (H56) or combined with different adjuvants (o/w squalene, CAF01, alum, and CpG), and spleens were collected 7 days after immunization. Cytokine secretion was detected upon 3 days of H56 antigen restimulation by Luminex immunoassay. Values, expressed as picograms per milliliter, are reported as the arithmetic mean ± SD of H56-stimulated minus the respective unstimulated samples, of five animals per group.